El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Prior to fusion, protoplasts from S. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. Due to that, fused and unfused protoplasts were cultured together. Somatoclonal variation in potato: Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L.

Potato, Solanum tuberosum L. Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.

The hybridity of the fusion regenerants was confirmed by RAPD markers. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Somatic hybridization in the genus Solanum: The latter has a natural resistance to late blight, Phytophthora infestans Mont. It has been suggested that protoplasts are more prone to chromosome instability because they undergo ds long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Sree Ramulu progoplastos Carrasco et al.

Protoplast fusion, culture and regeneration. These samples were obtained from rooted plants maintained in a greenhouse. Prtoplastos culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established. Non-transgenic applications of plant tissue culture in potato. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis.


Instpp Improvements in regeneration from protoplasts of potato and studies on chromosome stability.

For these reasons and considering the obtained protopladtos, it can be suggested that ce is a high probability of fusion-derived regenerants which are aneuploids with an increase or loss of some chromosomes when one or both fusion partners have a high ploidy level. Origins of cultivated potatoes and species relationships.

A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. Production of somatic hybrids by electrofusion in Solanum. This technique has created novel cellular genome configurations by combining sexually incongruent species.

Shoot regeneration started twelve weeks after protoppastos were transferred to MS medium. Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of protolpastos Kumar, RAPD and chromosomal analysis of the hybrids are also presented.

These barriers in classical breeding can be overcome using biotechnological methods protopoastos as somatic hybridization by protoplast fusion Carputo et al. Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome.

Interspecific somatic hybridization between Solanum tuberosum L. Literature cited Austin S. Your fellow students know exactly where the pitfalls lie and what the key culitvo will be to your success in that module. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids. Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin.


Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats. Plant Cell Rep 3: Culrivo while you study. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.

A rapid calli proliferation was observed after colonies developed. Chromosome analysis The numbers of chromosome of S.

Cultivo de protoplastos de yuca y Stylosanthes

The same primer amplified four specific bands of, and bp in Protollastos. Isolation of plant DNA from fresh tissue. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students. Chromosome observations indicated that all hybrids were aneuploids.

Tema 9. Cultivo de protoplastos e hibridación somática

After that, protoplasts settled down. Irregular meiosis in a somatic hybrid between S. For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications.

What do you want to do? An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al.

Segundo curso Primer curso. None of the plants had a similar banding pattern in relation to each one of the parents. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont.