ANTIBACTERIAL ACTIVITY OF ADHATODA VASICA PDF

Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. Separation was carried out at room temperature.

Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica. The following Gram negative, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line.

Vasicine showed antimicrobial activity against S. Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ].

Conflict of Interests The authors declare that there is no conflict vasifa interests regarding the publication of this paper. BHT is used as standard of antioxidant. In the present communication we report the antimicrobial, antioxidant, and anticancer effects of Vasicine acetate acetylated from Vasicine obtained from A. Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M.

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The solutions were filtered through a membrane filter pore size 0. The chemicals are given in delta scale with TMS as an internal standard. Effect of Adhatoda vasica massage in pyorrhoea. Antitussive effect of Adhatoda vasica extract on mechanical or chemical stimulation-induced coughing in animals. Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. Antimicrobial Activity Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ].

A voucher specimen No. Estimates of worldwide burden of cancer in Ignacimuthu123 C. Streptomycin was used as positive control for bacteria and Ketoconazole as positive control for fungi. The worldwide threat of antimicrobial resistance.

Conclusion Vasicine acetate was obtained by acetylation of Vasicine recovered from A. Balachandran2 S. The compound showed prominent cytotoxic activity in vitro against A lung adenocarcinoma cancer cell line. Vasicine is reported to have bronchodilatory, respiratory stimulant, and uterine stimulant effects [ 14 ].

The structure of the isolated compound Vasicine and Vasicine axtivity Figure 1. Published online Jan The plant contains alkaloids such as Vasicine, antiacterial, deoxyvasicine, vasicol, adhatodinine, and vasicinol [ 12 ]. The MIC for fungi was defined as the lowest extract concentration, showing no visible fungal growth after incubation time.

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In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Clinical and Laboratory Standards Institute; The extract was filtered through Whatman No. Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m.

The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. BoxRiyadhSaudi Arabia. Minimum Inhibitory Concentration MIC Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].

It was then distilled on a water bath and the residue was washed with little n-hexane. Acanthaceae is used in the indigenous system of medicine in India. Cytotoxic A and antimicrobial effects of Methylobacterium sp.

Antibacterial activity of adhatoda vasica leaf extract

All melting point values are uncorrected and taken by open capillary method on a heating block Instrument. Council of Scientific and Industrial Research; The plant is reported to show abortifacient [ 7 ], antimicrobial [ 89 ], and antitussive activities [ 10 ]. Minimum inhibitory concentration of Vasicine acetate against tested bacteria and fungi. Vasicine acetate was obtained by acetylation of Vasicine.